Rearing Frames

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Queen rearing frames are special frames hung in the hive at different stages of queen rearing: the grafting (cell-bar) frame on which the queen cells are lined up, the restriction frame that confines the breeder queen to a single comb, the holding frame that protects ripe queen cells, and the queen bank frame that stores caged queens. All of them must fit the standard size of the hive and be easy to remove and insert without harming the cells or the queens. This page covers the types of frames, how they are built and chosen, and how they are used and maintained.

What is a queen rearing frame?

In queen rearing, queen cells, larvae and queens must sit inside the hive in an orderly, traceable and easily accessible way. The frames used for this look like ordinary hive frames from the outside, but instead of comb they contain bars, cell holders, excluder surfaces or cage slots. Because the frame is standard size, it can easily be hung in the middle of a starter, finisher or mating colony, where the nurse bees are most concentrated.

Only one of these frames, the restriction frame, holds comb for the queen to lay in. The grafting frame has no comb; the queen cups are lined up on bars and the larvae are transferred from another comb.

Which frames are used in queen rearing?

FrameConstructionStage where it is used
Grafting (cell-bar) frame2–3 horizontal bars inside; queen cups or cell holders on the barsRearing queen cells in the starter and finisher; royal jelly production
Restriction frame (frame cage)A box enclosing one comb, with sides made of queen excluderObtaining larvae of the same age in the breeder colony
Holding (emergence cage) frameA bar or slot arrangement holding each ripe queen cell in its own cageDelayed distribution, collecting virgin queens
Queen bank frameSlots in which many shipping cages are lined upShort-term storage of mated queens
Mini mating frameSmall plastic or wooden frame made specifically for mini boxesMating

How is a grafting frame built and chosen?

A grafting frame is a frame with the same outer dimensions as your hive's brood frame, but with no comb inside. Two or three cell bars are fitted horizontally between the side bars.

  • Removable or rotating bar: If the bar can be taken out of its slot or turned on its own axis, the mouths of the cups can be turned upwards for comfortable work during grafting and then turned back.
  • Spacing between bars: Leave enough space so that the cells on the lower bar do not touch the bar above or the bottom of the frame; ripe queen cells are long.
  • Cup spacing: Cups are spaced along the bar so that they will not stick to each other once they mature. Cells set too close together get damaged when they are separated.
  • Cell holders: Plastic holders fit onto the bar and the cup sits on top of the holder. Because the ripe cell is removed together with its holder, the risk of crushing it is reduced.
  • For wax cups: Wax cups are fixed to the bar with a drop of molten wax; the wax must be clean and free of treatment residues.
  • Label space: Leave space on the top bar to write the batch number and grafting date.
Yellow cell holders and plastic queen cups lined up on two metal bars in a wooden frame
A cell-bar frame ready for grafting: yellow cell holders with plastic queen cups beneath them are lined up on two metal bars. Enough space is left between the bars for the ripe queen cells to lengthen. Photo: APHA (National Bee Unit), Crown Copyright · Open Government Licence v3.0 (Crown Copyright)
A single cell bar carrying ripe queen cells with their caps opened
A single cell bar taken out of the frame; the queens have emerged and the tips of the cells have opened like lids. A removable bar makes it easier to distribute the cells one by one. Photo: Disgwylfa / Wikimedia Commons · CC BY 2.0

How is a grafting frame used in the hive?

  1. Let the bees clean the cups: The prepared frame is placed in a colony one day before grafting; the bees clean the new plastic and give it the colony scent.
  2. Graft: The bars are taken out or turned one at a time, grafted, put back in the frame and covered with a damp cloth.
  3. Give to the starter: The frame is hung in the middle of the starter, next to a pollen frame.
  4. Move to the finisher: About 24 hours later acceptance is checked; without jarring, the frame is moved to the upper box of the finisher, between open brood and a pollen frame.
  5. Distribute: On day 10 after grafting the ripe queen cells are removed together with their holders.
Cell-bar frame on which nurse bees are drawing out queen cells from plastic cups
Queen cells developing in the finisher: nurse bees are completing the queen cells by extending wax over the plastic cups on two bars. Photo: APHA (National Bee Unit), Crown Copyright · Open Government Licence v3.0 (Crown Copyright)

Common mistakes with grafting frames

  • Loose holders: A holder that sits loosely on the bar can turn or fall off when the frame is lifted.
  • Wrong size: A frame that does not sit properly in the hive leads the bees to attach the queen cells to the neighbouring comb.
  • Jarring capped cells: In the days after capping the frame is not laid flat or shaken; bees are removed with a brush.

What is a restriction frame (frame cage) for?

A restriction frame is a box that encloses one comb and whose sides are made of queen excluder. The breeder queen is placed in this cage together with an empty, light-coloured comb and kept there for one or two days. Worker bees can pass through the excluder and tend the eggs, but the queen cannot leave the comb. As a result, all the eggs on the comb are the same age and the grafting day can be calculated precisely.

The same cage is also used in varroa control to restrict the queen's laying temporarily; but in the context of queen rearing its main job is pinning down the age of the larvae.

Holding frame for ripe queen cells

On days 9–10 after grafting, protective cages can be fitted over the ripe queen cells. These cages sit on the bars of the grafting frame or in the slots of a separate holding frame. Even if a queen emerges early she stays in her own cage and cannot reach the other cells, which gives one or two days of flexibility for distribution.

  • The holding frame is kept above the queen excluder in a queenright colony, or in a queenless, populous colony.
  • So that an emerging queen can feed inside the cage, the cage should contain a little fondant or be designed so that the bees can feed her through the mesh.
  • A virgin queen that emerges in the cage is not kept there for days; she is given to a mating nucleus as soon as possible.
Perforated plastic protective cages fitted over ripe queen cells on a cell-bar frame
Perforated plastic protective cages being slipped over ripe queen cells on a cell-bar frame. The cages prevent a queen that emerges early from destroying the other cells. Photo: Conall from Downpatrick, Northern Ireland / Wikimedia Commons · CC BY 2.0
Wooden queen emergence cages hung between the frames in a honey super
Wooden emergence cages hung between the frames in the upper box of the hive. Each cage holds one ripe queen cell; the emerging queen waits in the cage under the care of the bees. Photo: Maja Dumat from Deutschland (Germany) / Wikimedia Commons · CC BY 2.0

Queen bank frame

A queen bank means lining up mated queens, taken from mating nuclei or prepared for sale, in shipping cages on a frame and leaving them in the care of a strong colony. The frame has slots or laths to hold the cages; the cages are arranged with their mesh sides open to the bees.

  • Colony: Choose a well-fed colony rich in young bees; queenless colonies, or colonies whose queen is kept in another box behind an excluder, are used.
  • Duration: The bank is for short-term storage; the queens are given to colonies at the first opportunity.
  • Checks: Check the bank colony regularly for queen cells being raised and for dead queens in the cages.

Maintenance, cleaning and storage

  • Cleaning: Bars, holders and cages are cleared of wax and propolis after each batch; plastic parts are washed in hot water.
  • Odour: Parts are not stored alongside varroa treatments, fuel or insecticides; odours lower acceptance.
  • Disease: Frames and cages used in a colony showing signs of disease are not given to another colony without cleaning.
  • Winter storage: Frames are stored in a dry, enclosed place, protected from wax moth.
Make it a habit to write the batch number and grafting date on the top bar of every frame. When several batches are running at once, an undated frame can make you miss the distribution day and lose the whole batch.

Frequently asked questions

How many bars go in a grafting frame?

Usually two or three. The more bars there are, the narrower the gaps; the key point is that ripe queen cells must not touch each other or the bar below.

Can a grafting frame be made from an ordinary frame?

Yes. It can be made by adding two or three horizontal laths, resting on the side bars, to an empty brood frame from your hive. Removable laths make grafting easier.

How many days is the queen kept in a restriction frame?

One or two days is usually enough. Keeping her longer has a negative effect on the queen and on the colony's routine.

What is a queen bank?

It is the short-term storage of caged, mated queens lined up on a frame in the care of a strong colony; the queens are given to colonies at the first opportunity.

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