Pollen Traps

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Besides production, a pollen trap is also used as a measuring and monitoring tool: the amount, colour and composition of pollen collected at short, regular intervals show which plants the bees are working, how strong the source is and what pesticide risk exists around the apiary. This page explains how to choose a trap for monitoring, how to standardise sampling and how to interpret the results.

What does a pollen trap do as a monitoring tool?

The hive entrance is a mirror of the vegetation around the apiary. The pellets that fall into the trap drawer are a concrete sample of where the foragers flew that day. For production a trap runs for days; for monitoring, short and regular sampling windows are enough. In this way comparable data are collected all year while the colony's protein intake is barely affected.

  • Tracking the pollen flow: Shows in which weeks pollen is plentiful and in which it is scarce; lets you draw up your own apiary's pollen calendar.
  • Identifying the plant source: Pellet colour gives a rough clue; microscopic examination gives definite information.
  • Comparing apiary sites: The amount and diversity of pollen collected by colonies at different sites in the same period can be compared.
  • Monitoring residues: Pollen samples are among the best materials for laboratory testing of the agricultural pesticides the bees are exposed to.
Foragers with orange pollen pellets at the hive entrance
Foragers carrying pollen at the entrance. The trap sets aside part of these pellets as a sample. Photo: uacescomm / Wikimedia Commons · CC BY-SA 2.0

Which pollen trap should you choose for monitoring?

The main aim in measurement is comparability. So it matters less that the trap is highly efficient than that it works the same way every time.

  • Same model: Use the same type of trap with the same hole size on all the hives to be compared. Different models strip off pollen at different rates.
  • Drawer that empties completely: A flat-bottomed drawer that is easy to pull out and leaves no pollen in its corners ensures the sample is taken in full.
  • Mesh cover: Fine mesh above the drawer stops bees reaching the fallen pollen and taking it back; without the mesh the measurement comes out short.
  • On–off mechanism: A trap that can be disabled while the grid stays on the hive can be left in place between sampling days without disturbing the colony.
  • Protection from rain and sun: A sample that gets wet or sits in the sun deteriorates, and its weighing and analysis become unreliable.
Perforated plate of a pollen trap, stripped pollen pellets and the mesh below
Front of the trap plate: bees passing through the holes, the stripped pellets and, below, the mesh that stops the bees reaching the pollen. Photo: Matteogiusti / Wikimedia Commons · CC BY-SA 3.0

How do you take pollen samples step by step?

  1. Choose colonies: Choose several healthy colonies of similar strength, ideally with queens of the same age. Data from a single colony can be misleading.
  2. Fix the schedule: For example, run the trap one day a week, from morning to evening or for exactly 24 hours. Use the same duration for every measurement.
  3. Collect and weigh: Empty the drawer at the end of the window and record the weight of the fresh pollen. Weighing should be done the same day, on the same scale.
  4. Take a subsample: The pollen is mixed thoroughly and a subsample of a set weight is taken; this is sorted by colour and the share of each colour group is weighed.
  5. Keep records: Note the date, time window, weather (temperature, wind, rain), colony number and the flowering observed.
  6. Freeze the analysis sample: The part going to the laboratory is put in a clean, labelled container, frozen immediately and delivered without breaking the cold chain.
  7. Close the trap: When sampling is finished the trap is disabled; the colony goes on carrying in its pollen normally.
Pollen pellets of different colours in a trap drawer
A one-day trap sample: the range of colours shows the bees working many sources at the same time. Photo: APHA (National Bee Unit), Crown Copyright · Open Government Licence v3.0 (Crown Copyright)
Pollen pellets ready to be sorted by colour
Dried pollen pellets. The subsample is sorted by colour and the share of each group is weighed. Photo: beepollenhub / Wikimedia Commons · CC BY 2.0

How do you interpret pollen measurement results?

MeasurementWhat does it show?Limitation
Daily fresh pollen weightStrength of the pollen flow, differences between periodsDepends on weather and colony strength; suitable for relative comparison
Colour groups and their sharesDominant sources, diversity of the dietDifferent plants can produce pellets of the same colour
Microscopic (palynological) examinationSource at plant species or genus levelRequires expertise and a reference collection
Residue analysisAgricultural pesticides the bees are exposed toOnly done in a competent laboratory; the sample reflects its period

A trap strips off not all the incoming pollen but a variable part of it. This proportion varies with the trap model, bee race, pellet size and the state of the colony. So the measurements do not reflect the colony's total pollen intake, but they do meaningfully reflect comparisons made with the same set-up.

Scanning electron microscope image of different pollen grains in a single pellet
Electron microscope image of a single pollen pellet: pollen grains of different shapes can occur in the same pellet. Colour alone does not tell you the source for certain. Photo: Atalyssa / Wikimedia Commons · CC BY 4.0

How do you take a pollen sample for residue monitoring?

Bees can carry pollen from treated plants within their flight range. If colony losses or mass bee deaths in front of the hives are seen during spraying periods, a fresh pollen sample is valuable evidence for investigating exposure.

  • Use a food-grade container that can be labelled and has never been in contact with chemicals.
  • Keep the sample from each colony and each date separate; do not mix samples.
  • Freeze the sample immediately; a sample left at room temperature deteriorates and gives analysis errors.
  • If necessary, also take samples of dead bees and comb in separate containers; in cases of mass deaths, report to the official veterinary service / local agriculture authority.
Sample quantity and shipping conditions vary from laboratory to laboratory. Talk to the laboratory you will work with before taking the sample; a wrongly stored sample gives no result.

Does monitoring weaken the colony?

Sampling in short windows does not noticeably affect the colony, because the trap runs for only a few hours or a day. Even so, sampling frequency should be reduced for weak and newly split colonies and in periods critical for pollen, such as early spring and late summer. Leaving the trap permanently open for monitoring is unnecessary and reduces brood rearing.

Common mistakes

  • Comparing different trap models: You cannot tell whether a difference comes from the trap or the environment.
  • Changing the sampling time: Data from 6 hours one day and 24 hours another cannot be compared.
  • Treating colour as definite species information: Colour is only a clue.
  • Letting the analysis sample sit: Residue and microbiology results lose their meaning.
  • Not recording the weather: The low value of a rainy day may be mistaken for a dearth.

Frequently asked questions

How long should the trap run for monitoring?

Anywhere from a few hours to 24 hours is enough, provided the duration is the same for every measurement. What matters is that the duration and schedule stay fixed.

Can I tell the plant from the pollen colour?

You can make a rough guess, but different plants can produce pellets of similar colour. Definite information requires microscopic examination.

How do you detect pesticide residues in a pollen sample?

Only by laboratory analysis. The sample should reach the laboratory in a clean container, labelled and frozen.

How many hives should be measured?

A single hive can be misleading; several colonies of similar strength give a more reliable average.

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