Varroa Treatment Methods

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⚠️ Important: The information on this page is for general guidance only and does not replace diagnosis or treatment. For a definite diagnosis, always consult a veterinarian or have samples tested by an authorised laboratory. Use only licensed medicines, following the label and the legal withdrawal periods. If you suspect a notifiable disease, inform the official veterinary service.

Successful varroa control does not come from a single medicine but from a year-round programme: regular counts, treatment at the right time, rotating methods and measuring the result again. The most critical periods are late summer, when the winter bees are reared, and early winter, when brood decreases or stops.

Measure first: how to determine the mite load

Judging by eye is not enough; by the time mites are visible on bees, the load is usually already high. Base your decision on counts.

  • Alcohol (or soapy water) wash: About 300 bees (roughly half a glass) taken from a brood frame are shaken in a container and the dislodged mites are counted. This is the most reliable field method; the bees die.
  • Icing sugar shake: The same sample is shaken with icing sugar and the mites are shaken out through a mesh. The bees can be returned to the hive; the result may be slightly lower than with a wash.
  • Natural mite drop tray: An oiled or sticky tray is placed under the mesh floor and the drop over several days is converted into a daily average. Useful for following trends and seeing the effect of a treatment.
  • Uncapping drone brood: Capped drone cells are opened with a fork and the pupae are checked for mites; this gives an early warning in spring.

Commonly used action thresholds: in summer and autumn, about 3 mites per 100 bees in a wash; lower in spring and in the broodless period (1–2 mites per 100 bees). If the official recommendation in your region is different, follow it.

Varroa mite on the thorax of a drone standing on capped brood
A female varroa mite clinging to a bee's thorax. Mites on bees are counted using the wash or icing sugar method. Photo: Waugsberg / Wikimedia Commons · CC BY-SA 4.0
Many dark varroa mites in rows on a mite drop tray
Mite drop tray: hundreds of dark brown mites that have fallen beneath the spaces between the frames. The tray is used both for monitoring and for seeing the effect of a treatment. Photo: APHA (National Bee Unit), Crown Copyright · Open Government Licence v3.0 (Crown Copyright)
Red-brown mites on drone pupae removed with a fork
Drone pupae removed with a fork: the red-brown dots on the white pupae are varroa mites. Photo: APHA (National Bee Unit), Crown Copyright · Open Government Licence v3.0 (Crown Copyright)

Groups of methods

GroupExampleStrengthLimitation
Organic acidsOxalic, formic, lactic acidNo known resistance; low risk of persistent residues in honey when used correctlyOxalic and lactic acid do not reach mites in capped brood; formic acid is very sensitive to temperature
Essential oil componentsThymolNo resistance reported; can be used in organic beekeepingTemperature-dependent; can leave an odour and taste in honey; not used while supers are on
Synthetic acaricidesAmitraz; pyrethroids (flumethrin, tau-fluvalinate)Easy-to-use strip formulations; effective throughout the brood periodResistance develops; fat-soluble substances can accumulate in wax
Biotechnical methodsDrone brood removal, brood break, artificial swarm, trap combNo medicines; no resistanceLabour-intensive; usually not enough on their own

Organic acids

Formic acid is the only organic acid that can also act inside capped brood cells, so it can be used while brood is present. However, in great heat it evaporates quickly and can cause brood and queen losses, while in the cold its effect may be insufficient; strictly follow the temperature range on the product label.

Oxalic acid kills only the mites on the bees. It is very effective in the broodless period; when brood is present, a single application is not enough. It is applied by trickling or sublimation. Lactic acid, on the other hand, is sprayed onto the bees on the frames; it is suitable for swarms, package bees and small colonies. Details are on the relevant pages.

Synthetic acaricides

Authorised strip products are effective and practical; but repeating the same active substance year after year selects for resistant generations of mites. Resistance to amitraz and pyrethroids has been reported in many parts of the world. Flumethrin and tau-fluvalinate belong to the same group; if there is resistance to one, the other usually does not work either.

Acaricide strip hung between two frames in a hive
A synthetic acaricide strip hung between the frames in the brood area, where the bees are most concentrated. Photo: APHA (National Bee Unit), Crown Copyright · Open Government Licence v3.0 (Crown Copyright)

Biotechnical methods

  • Drone brood removal: The mite prefers drone brood cells. In spring, an empty or shallow frame is given; once the drone brood built on it is capped, it is cut out and discarded before the bees emerge.
  • Brood break: The queen is caged or the colony is split; once the capped brood has emerged, all the mites are on the bees and a single oxalic acid treatment is very effective.
  • Trap comb: The queen is confined to a single frame at set intervals; the mites enter the brood on this frame, and the frame is removed after it has been capped.
Capped drone brood comb cut from the lower part of a frame
Drone brood removal: capped drone brood built at the bottom of the frame is cut out and removed from the hive. Photo: APHA (National Bee Unit), Crown Copyright · Open Government Licence v3.0 (Crown Copyright)

Annual programme (sample outline)

  1. Spring: Do a count; start drone brood removal. If the load is high, treat with a suitable method before the supers go on.
  2. Summer (nectar flow): No medicines are applied while supers are on. Continue the biotechnical methods and count once a month.
  3. Late summer (immediately after the harvest): The main treatment of the year. As the winter bees are reared at this time, delay means losing a healthy generation of winter bees.
  4. Early winter (broodless period): Oxalic acid to clear the remaining mites. In regions with mild winters brood may not stop; take this into account before opening the hive.
  5. After every treatment: Count again within a few weeks. If the expected drop has not occurred, treatment error, reinfestation or resistance should be investigated.
Oxalic acid being trickled from above onto the bees of the winter cluster
Oxalic acid being trickled between the frames onto the clustered bees during the broodless winter period. Photo: APHA (National Bee Unit), Crown Copyright · Open Government Licence v3.0 (Crown Copyright)

Rules for treatment

  • Use only veterinary products authorised in your country; follow the amount, duration and temperature conditions on the label. Underdosing and strips left in too long speed up resistance.
  • Do not treat while supers are on; observe the withdrawal period on the label.
  • Rotate the groups of active substances from year to year.
  • Do not put livestock medicines, unauthorised mixtures or home-made strips into the hive; they are the main source of honey residues and resistance problems.
  • Treat all colonies in the same apiary at the same time; coordinate with neighbouring beekeepers if possible. Robbing and drifting carry mites back into a hive that has been cleared.
  • Record the product, date, duration and count result for each hive.
Young bees with deformed wings, perforated and irregular capped brood, and bees unable to fly in front of the hive are signs of an advanced varroa infestation and the viruses that accompany it. When these signs appear, the intervention is already late; start the programme earlier next year.
Bees with crumpled, short wings on the comb
Deformed wing virus associated with a high varroa load: the young bee in the centre has crumpled, short, useless wings; the capped brood is also irregular and perforated. Photo: APHA (National Bee Unit), Crown Copyright · Open Government Licence v3.0 (Crown Copyright)
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